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enterococcus gallinarum  (MedChemExpress)


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    MedChemExpress enterococcus gallinarum
    Enterococcus Gallinarum, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/enterococcus+gallinarum/pm42046343-43-18-32?v=MedChemExpress
    Average 94 stars, based on 3 article reviews
    enterococcus gallinarum - by Bioz Stars, 2026-07
    94/100 stars

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    enterococcus gallinarum  (ATCC)
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    PSC bacterial supernatants affect human cell viability. Colonic (Caco-2), biliary (EGI-1), and hepatic (HepG2) human cancer cells were treated with a 1:3 or 1:1 ratio (vol/vol) of bacterial culture supernatant for 16 h under anaerobic conditions. BHI+ medium was used as a control. B. fragilis and E. <t>gallinarum</t> were used as reference strains and were not derived from PSC patients, as indicated by striped bars. The following isolates were used in these experiments : E. faecalis _PSC06_col01, E. coli _PSC10_col01, F. necrophorum _PSC08_col02, K. pneumoniae _PSC09_col49 (H 2 S producer), K. pneumoniae _PSC03_col01 (non-producer), S. salivarius _PSC01_col01, V. dispar _PSC07_col14, and V. parvula _PSC07_col05. Two-way ANOVA was performed followed by Dunnett’s multiple comparisons test using Graphpad Prism 10 software (values are shown as mean ± SD; four biological replicates; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
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    PSC bacterial supernatants affect human cell viability. Colonic (Caco-2), biliary (EGI-1), and hepatic (HepG2) human cancer cells were treated with a 1:3 or 1:1 ratio (vol/vol) of bacterial culture supernatant for 16 h under anaerobic conditions. BHI+ medium was used as a control. B. fragilis and E. gallinarum were used as reference strains and were not derived from PSC patients, as indicated by striped bars. The following isolates were used in these experiments : E. faecalis _PSC06_col01, E. coli _PSC10_col01, F. necrophorum _PSC08_col02, K. pneumoniae _PSC09_col49 (H 2 S producer), K. pneumoniae _PSC03_col01 (non-producer), S. salivarius _PSC01_col01, V. dispar _PSC07_col14, and V. parvula _PSC07_col05. Two-way ANOVA was performed followed by Dunnett’s multiple comparisons test using Graphpad Prism 10 software (values are shown as mean ± SD; four biological replicates; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Journal: mSphere

    Article Title: Cultured bacteria isolated from primary sclerosing cholangitis patient bile induce inflammation and cell death

    doi: 10.1128/msphere.00550-25

    Figure Lengend Snippet: PSC bacterial supernatants affect human cell viability. Colonic (Caco-2), biliary (EGI-1), and hepatic (HepG2) human cancer cells were treated with a 1:3 or 1:1 ratio (vol/vol) of bacterial culture supernatant for 16 h under anaerobic conditions. BHI+ medium was used as a control. B. fragilis and E. gallinarum were used as reference strains and were not derived from PSC patients, as indicated by striped bars. The following isolates were used in these experiments : E. faecalis _PSC06_col01, E. coli _PSC10_col01, F. necrophorum _PSC08_col02, K. pneumoniae _PSC09_col49 (H 2 S producer), K. pneumoniae _PSC03_col01 (non-producer), S. salivarius _PSC01_col01, V. dispar _PSC07_col14, and V. parvula _PSC07_col05. Two-way ANOVA was performed followed by Dunnett’s multiple comparisons test using Graphpad Prism 10 software (values are shown as mean ± SD; four biological replicates; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Article Snippet: Enterococcus gallinarum (gift from Martin Kriegel at Yale School of Medicine) ( ) and Bacteroides fragilis (ATCC 25285) were used as reference strains and were not derived from PSC patients.

    Techniques: Control, Derivative Assay, Software

    Bacterial supernatants from the PSC patient isolates E. faecalis and V. parvula increased epithelial permeability. A differentiated monolayer of Caco-2 cells was treated with a 1:1 ratio (vol/vol) of HBSS buffer to bacterial culture supernatant for 6 or 12 h under anaerobic conditions. BHI+ medium was used as a control. B. fragilis and E. gallinarum were used as reference strains and were not derived from PSC patients, as indicated by striped bars. The following isolates were used in these experiments : E. faecalis _PSC06_col01, E. coli _PSC10_col01, F. necrophorum _PSC08_col02, K. pneumoniae _PSC09_col49 (H 2 S producer), K. pneumoniae _PSC03_col01 (non-producer), S. salivarius _PSC01_col01, V. dispar _PSC07_col14, and V. parvula _PSC07_col05. Two-way ANOVA was performed followed by Dunnett’s multiple comparisons test using Graphpad Prism 10 software (values are shown as mean ± SD; four biological replicates; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Journal: mSphere

    Article Title: Cultured bacteria isolated from primary sclerosing cholangitis patient bile induce inflammation and cell death

    doi: 10.1128/msphere.00550-25

    Figure Lengend Snippet: Bacterial supernatants from the PSC patient isolates E. faecalis and V. parvula increased epithelial permeability. A differentiated monolayer of Caco-2 cells was treated with a 1:1 ratio (vol/vol) of HBSS buffer to bacterial culture supernatant for 6 or 12 h under anaerobic conditions. BHI+ medium was used as a control. B. fragilis and E. gallinarum were used as reference strains and were not derived from PSC patients, as indicated by striped bars. The following isolates were used in these experiments : E. faecalis _PSC06_col01, E. coli _PSC10_col01, F. necrophorum _PSC08_col02, K. pneumoniae _PSC09_col49 (H 2 S producer), K. pneumoniae _PSC03_col01 (non-producer), S. salivarius _PSC01_col01, V. dispar _PSC07_col14, and V. parvula _PSC07_col05. Two-way ANOVA was performed followed by Dunnett’s multiple comparisons test using Graphpad Prism 10 software (values are shown as mean ± SD; four biological replicates; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Article Snippet: Enterococcus gallinarum (gift from Martin Kriegel at Yale School of Medicine) ( ) and Bacteroides fragilis (ATCC 25285) were used as reference strains and were not derived from PSC patients.

    Techniques: Permeability, Control, Derivative Assay, Software

    PSC bacterial supernatants induced transcription of inflammatory cytokines in biliary and hepatic cells. Biliary (EGI-1) and hepatic (HepG2) cells were treated with a 1:1 ratio (vol/vol) of bacterial culture supernatant to cell culture medium for 6 h under anaerobic conditions, and the effect on transcription of target genes was assessed by qPCR. BHI+ medium was used as a control. E. gallinarum was used as a reference strain and was not derived from PSC patients, as indicated by striped bars. Results were normalized to UBC mRNA expression. The following isolates were used in these experiments : E. faecalis _PSC06_col01, E. coli _PSC10_col01, F. necrophorum _PSC08_col02, K. pneumoniae _PSC09_col49 (H 2 S producer), K. pneumoniae _PSC03_col01 (non-producer), S. salivarius _PSC01_col01, V. dispar _PSC07_col14, and V. parvula _PSC07_col05. One-way ANOVA was performed followed by Dunnett’s multiple comparisons test using Graphpad Prism 10 software (values are shown as mean ± SD; four biological replicates from independent experiments with three technical replicates each; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Journal: mSphere

    Article Title: Cultured bacteria isolated from primary sclerosing cholangitis patient bile induce inflammation and cell death

    doi: 10.1128/msphere.00550-25

    Figure Lengend Snippet: PSC bacterial supernatants induced transcription of inflammatory cytokines in biliary and hepatic cells. Biliary (EGI-1) and hepatic (HepG2) cells were treated with a 1:1 ratio (vol/vol) of bacterial culture supernatant to cell culture medium for 6 h under anaerobic conditions, and the effect on transcription of target genes was assessed by qPCR. BHI+ medium was used as a control. E. gallinarum was used as a reference strain and was not derived from PSC patients, as indicated by striped bars. Results were normalized to UBC mRNA expression. The following isolates were used in these experiments : E. faecalis _PSC06_col01, E. coli _PSC10_col01, F. necrophorum _PSC08_col02, K. pneumoniae _PSC09_col49 (H 2 S producer), K. pneumoniae _PSC03_col01 (non-producer), S. salivarius _PSC01_col01, V. dispar _PSC07_col14, and V. parvula _PSC07_col05. One-way ANOVA was performed followed by Dunnett’s multiple comparisons test using Graphpad Prism 10 software (values are shown as mean ± SD; four biological replicates from independent experiments with three technical replicates each; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Article Snippet: Enterococcus gallinarum (gift from Martin Kriegel at Yale School of Medicine) ( ) and Bacteroides fragilis (ATCC 25285) were used as reference strains and were not derived from PSC patients.

    Techniques: Cell Culture, Control, Derivative Assay, Expressing, Software

    PSC bacterial supernatants affected transcription of genes related to sulfur metabolism, mucin expression, and the bicarbonate umbrella in biliary and hepatic cells. Biliary (EGI-1) and hepatic (HepG2) cells were treated with a 1:1 ratio (vol/vol) of bacterial culture supernatant to cell culture medium for 6 h under anaerobic conditions, and the effect on transcription of target genes was assessed by qPCR. BHI+ medium was used as a control. E. gallinarum was used as a reference strain and was not derived from PSC patients, as indicated by striped bars. Results were normalized to UBC mRNA expression. The following isolates were used in these experiments : E. faecalis _PSC06_col01, E. coli _PSC10_col01, F. necrophorum _PSC08_col02, K. pneumoniae _PSC09_col49 (H 2 S producer), K. pneumoniae _PSC03_col01 (non-producer), S. salivarius _PSC01_col01, V. dispar _PSC07_col14, and V. parvula _PSC07_col05. One-way ANOVA was performed followed by Dunnett’s multiple comparisons test using Graphpad Prism 10 software (values are shown as mean ± SD; four biological replicates from independent experiments with three technical replicates each; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Journal: mSphere

    Article Title: Cultured bacteria isolated from primary sclerosing cholangitis patient bile induce inflammation and cell death

    doi: 10.1128/msphere.00550-25

    Figure Lengend Snippet: PSC bacterial supernatants affected transcription of genes related to sulfur metabolism, mucin expression, and the bicarbonate umbrella in biliary and hepatic cells. Biliary (EGI-1) and hepatic (HepG2) cells were treated with a 1:1 ratio (vol/vol) of bacterial culture supernatant to cell culture medium for 6 h under anaerobic conditions, and the effect on transcription of target genes was assessed by qPCR. BHI+ medium was used as a control. E. gallinarum was used as a reference strain and was not derived from PSC patients, as indicated by striped bars. Results were normalized to UBC mRNA expression. The following isolates were used in these experiments : E. faecalis _PSC06_col01, E. coli _PSC10_col01, F. necrophorum _PSC08_col02, K. pneumoniae _PSC09_col49 (H 2 S producer), K. pneumoniae _PSC03_col01 (non-producer), S. salivarius _PSC01_col01, V. dispar _PSC07_col14, and V. parvula _PSC07_col05. One-way ANOVA was performed followed by Dunnett’s multiple comparisons test using Graphpad Prism 10 software (values are shown as mean ± SD; four biological replicates from independent experiments with three technical replicates each; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Article Snippet: Enterococcus gallinarum (gift from Martin Kriegel at Yale School of Medicine) ( ) and Bacteroides fragilis (ATCC 25285) were used as reference strains and were not derived from PSC patients.

    Techniques: Expressing, Cell Culture, Control, Derivative Assay, Software